The standard protocols for the purification of all known cytoskeleton proteins are presented in this manual. Proteins are listed alphabetically and each protocol follows a common format. Thus, the manual provides a quick and easy reference to all relevant procedures for cytoskeleton protein purification. The isolation procedure for each protein is shown in a clear flowchart, while the source of the protein, equipment and material needed, a list of suppliers, standard references, accession No. of sequences as well as further relevant facts and practical tips are given on a separate page.
Actomyosin by Akademie der Wissenschaften und der Literatur (Mainz)
The student of biological science in his final years as an undergraduate and his first years as a graduate is expected to gain some familiarity with current research at the frontiers of his discipline. New research work is published in a perplexing diversity of publications and is inevitably con cerned with the minutiae of the subject. The sheer number of research journals and papers also causes confusion and difficulties of assimilation. Review articles usually presuppose a background knowledge of the field and are inevitably rather restricted in scope. There is thus a need for short but authoritative introductions to those areas of modern biological research which are either not dealt with in standard introductory text books or are not dealt with in sufficient detail to enable the student to go on from them to read scholarly reviews with profit. This series of books is designed to satisfy this need. The authors have been asked to produce a brief outline of their subject assuming that their readers will have read and remembered much of a standard introductory textbook of biology. This outline then sets out to provide by building on this basis, the conceptual framework within which modern research work is progressing and aims to give the reader an indication of the problems, both conceptual and practical, which must be overcome if progress is to be maintained.
Cytoskeletal Proteins—Advances in Research and Application: 2012 Edition is a ScholarlyEditions™ eBook that delivers timely, authoritative, and comprehensive information about Cytoskeletal Proteins. The editors have built Cytoskeletal Proteins—Advances in Research and Application: 2012 Edition on the vast information databases of ScholarlyNews.™ You can expect the information about Cytoskeletal Proteins in this eBook to be deeper than what you can access anywhere else, as well as consistently reliable, authoritative, informed, and relevant. The content of Cytoskeletal Proteins—Advances in Research and Application: 2012 Edition has been produced by the world’s leading scientists, engineers, analysts, research institutions, and companies. All of the content is from peer-reviewed sources, and all of it is written, assembled, and edited by the editors at ScholarlyEditions™ and available exclusively from us. You now have a source you can cite with authority, confidence, and credibility. More information is available at http://www.ScholarlyEditions.com/.
Cytoskeletal Proteins: Advances in Research and Application: 2011 Edition is a ScholarlyBrief™ that delivers timely, authoritative, comprehensive, and specialized information about Cytoskeletal Proteins in a concise format. The editors have built Cytoskeletal Proteins: Advances in Research and Application: 2011 Edition on the vast information databases of ScholarlyNews.™ You can expect the information about Cytoskeletal Proteins in this eBook to be deeper than what you can access anywhere else, as well as consistently reliable, authoritative, informed, and relevant. The content of Cytoskeletal Proteins: Advances in Research and Application: 2011 Edition has been produced by the world’s leading scientists, engineers, analysts, research institutions, and companies. All of the content is from peer-reviewed sources, and all of it is written, assembled, and edited by the editors at ScholarlyEditions™ and available exclusively from us. You now have a source you can cite with authority, confidence, and credibility. More information is available at http://www.ScholarlyEditions.com/.
The plasma membrane acts as both a boundary and a site of exchange between the outside and the inside of a cell. The cytoskeleton plays essential roles in delineating membrane protein distributions, in defining structural and functional domains in the plasma membrane, and in regulating membrane protein, and ultimately, cell function. This volume reviews the regulation of membrane protein distribution, organization, and function at the plasma membrane by the cytoskeleton. Discussions also include the roles of cytoskeleton in the structural and functional organization of membranes and membrane proteins with emphasis on key problems, the current status of understanding, experimental approaches, and future directions.
This monograph begins with a general description of the cytoskeleton in axonal development and pathology and then moves to more detailed descriptions of particular components, including microtubules and associated proteins, neurofilaments and interacting proteins, actin and its binding proteins, and glial fibrillary acidic protein. The later chapters focus on the functional significance of the neuronal cytoskeleton in axonal transport and its regulation in health and disease states. The Cytoskeleton of the Nervous System will encourage further development of unifying principles and stimulate new conceptual and technical approaches toward a better understanding of cytoskeleton functions in health and disease.
The internal structure of a cell can be affected by signals in the form of small molecules outside the cell. These changes can then alter the shape or adhesiveness of the cell. This volume centers particularly on one family of cellular proteins which transmit these signals, the Rho Ras-like GTPases, and examines their role in normal cellular processes and development. Also discussed are their roles in cancer formation and microbial pathogenesis.
This book describes a new family of bio-polymer gels made from cytoskeletal proteins - actin, microtubule, and tropomyosin. The importance of the gel state with multi-scale hierarchical structure is emphasized to utilize emergent functions in living organisms. Detailed protocol of gel preparation, specified method of structure investigation, and dynamic studies of self-organization, self-healing, synchronized oscillating, and autonomous motility functions are introduced together with biomimetic functions of synthetic hydrogels.
The presence/absence of gene families with central roles in endomembrane and cytoskeleton dynamics in a variety of eukaryotic taxa and an understanding of eukaryote phylogeny allow the cellular machineries present in the last common ancestor of eukaryotes to be accurately reconstructed. Such a reconstruction is fundamental in order to understand eukaryotic diversification, since this is the ancestral cell from which all diversity arose. This book discusses the evolutionary origin and diversification of eukaryotic endomembranes and cytoskeleton from a cell biological and comparative genomic perspective.
The Neuronal Cytoskeleton, Motor Proteins, and Organelle Trafficking in the Axon, a new volume in the Methods in Cell Biology series continues the legacy of this premier serial with quality chapters authored by leaders in the field. This volume covers research methods in neuronal cells, and includes sections on such topics as actin transport in axons and neurofilament transport. Covers an increasingly appreciated field in cell biology Includes both established and new technologies Contributed by experts in the field
The Par protein family and the LIM kinase-slingshot-cofilin complex of proteins are implicated in regulating neuronal development and differentiation, the study of which holds promise for applications of neurological disease pathologies and therapies. This research project focuses on proteins that affect the components of the neuronal cytoskeleton, primarily actin and tubulin, which form the cell's actin filaments and microtubules, respectively. The main proteins of interest were the LIM kinase-slingshot-cofilin complex. Downstream of well-established polarity proteins Par-3 and Par-6, this complex impacts neurite differentiation through the stabilization or destabilization of actin and tubulin. By transfecting young neurons with an excess of a particular wild-type protein or with a knockdown plasmid of a particular protein, it was determined that cofilin wild-type and LIM kinase wild-type caused little change in polarity development while cofilin knockdown and slingshot wild-type caused moderate changes and LIM kinase knockdown dramatically reduced the normal polarity phenotype. Rescue mechanisms were also studied. By co-transfecting proteins known to affect normal polarity expression with an excess of actin and tubulin, it was found that polarity defects were able to be rescued. Future research avenues include studying the molecular mechanisms of and identifying alternative proteins for polarity rescue.
This volume deals with aspects of the cytoskeleton in different cell types and also describe examples of changes in the cytoskeleton which occur during various pathological states. These studies bring the exciting area of cytoskeleton research into the domain of medical science.
Cell membranes by Society of General Physiologists. Symposium