Recognition receptors play a key role in the successful implementation of chemical and biosensors. Molecular recognition refers to non-covalent speci?c binding between molecules, one of which is typically a macromolecule or a molecular assembly, and the other is the target molecule (ligand or analyte). Biomolecular recognition is typically driven by many weak interactions such as hydrogen bo- ing, metal coordination, hydrophobic forces, van der Waals forces, pi-pi interactions and electrostatic interaction (due to permanent charges, dipoles, and quadrupoles) the polarization of charge distributions by the interaction partner leading to ind- tion and dispersion forces, and Pauli-exclusion-principle-derived inter-atomic repulsion, and a strong, “attractive” force arising largely from the entropy of the solvent and termed the hydrophobic effect. In recent years, there has been much progress in understanding the forces that drive the formation of such complexes, and how these forces are relate to the physical properties of the interacting molecules and their environment allows rational design of molecules and materials that interact in speci?c and desired ways. This book presents a signi?cant and up-to-date review of the various recognition elements, their immobilization, characterization techniques by a panel of dist- guished scientists. This work is a comprehensive approach to the recognition receptors area presenting a thorough knowledge of the subject and an effective integration of these receptors on sensor surfaces in order to appropriately convey the state-of the-art fundamentals and applications of the most innovative approaches.
Principles of Bacterial Detection: Biosensors, Recognition Receptors and Microsystems will cover the up-to-date biosensor technologies used for the detection of bacteria. Written by the world's most renowned and learned scientists each in their own area of expertise, Principles of Bacterial Detection: Biosensors, Recognition Receptors and Microsystems is the first title to cover this expanding research field.
Key features include: Self-assessment questions and exercises Chapters start with essential principles, then go on to addressmore advanced topics More than 1300 references to direct the reader to keyliterature and further reading Highly illustrated with 450 figures, including chemicalstructures and reactions, functioning principles, constructivedetails and response characteristics Chemical sensors are self-contained analytical devices thatprovide real-time information on chemical composition. A chemicalsensor integrates two distinct functions: recognition andtransduction. Such devices are widely used for a variety ofapplications, including clinical analysis, environment monitoringand monitoring of industrial processes. This text provides anup-to-date survey of chemical sensor science and technology, with agood balance between classical aspects and contemporary trends.Topics covered include: Structure and properties of recognition materials and reagents,including synthetic, biological and biomimetic materials,microorganisms and whole-cells Physicochemical basis of various transduction methods(electrical, thermal, electrochemical, optical, mechanical andacoustic wave-based) Auxiliary materials used e.g. synthetic and natural polymers,inorganic materials, semiconductors, carbon and metallicmaterials properties and applications of advanced materials (particularlynanomaterials) in the production of chemical sensors andbiosensors Advanced manufacturing methods Sensors obtained by combining particular transduction andrecognition methods Mathematical modeling of chemical sensor processes Suitable as a textbook for graduate and final year undergraduatestudents, and also for researchers in chemistry, biology, physics,physiology, pharmacology and electronic engineering, this bookisvaluable to anyone interested in the field of chemical sensors andbiosensors.
This book focuses on the state-of-the-art of biosensor research and development for specialists and non-specialists. It introduces the fundamentals of the subject with relevant characteristics of transducer elements, as well as biochemical recognition molecules. This book is ideal for researchers of nanotechnology, materials science and biophysics.
A survey of the principal features of sensors based on biological materials, this text discusses the different types of biosensors and the manner in which they are connected to a range of transducers. Applications to such fields as environmental science and medicine are also considered.
Discusses the use of chemical sensors and biosensors for process and environmental monitoring and for medical applications. Presents advances in enzyme- and antibody-based biosensors, including enzyme electrodes and optical immunosensors. Discusses advances in acoustic, optical, and electrochemical biosensors. Describes on-line and off-line monitoring techniques for the fermentation process.
General introduction to biosensors and recognition receptors -- Biomarkers in health care -- The use of nanomaterials and microfluidics in medical diagnostics -- SPR-based biosensor technologies in disease detection and diagnostics -- Piezoelectric-based biosensor technologies in disease detection and diagnostics -- Electrochemical-based biosensor technologies in disease detection and diagnostics -- MEMS-based cell counting methods -- Lab-on-a-chip platforms for disease detection and diagnosis -- Applications of quantum dots in biosensors and diagnostics -- Applications of molecularly imprinted nanostructures in biosensors and diagnostics -- Smart nanomaterial's : applications in biosensors and diagnostics -- Applications of magnetic nanomaterial's in biosensors and diagnostics -- Graphene applications in biosensors and diagnostics -- Optical biosensors and applications to drug discovery for cancer cases -- Biosensors for detection of anticancer drug-DNA interactions
Despite achievements in the application of enzymes, antibodies and biological receptors to diagnostics and sensing, the last two decades have also witnessed the emergence of a number of alternative technologies based on synthetic chemistry. This volume shows how synthetic receptors can be designed with characteristics that make them attractive alternatives to biological molecules in the sensory and diagnostics fields, with contributions from leading experts in the area. Subjects covered include synthetic receptors for a range of biomolecules, the use of antimicrobial peptides for the detection of pathogenic microorganisms, the development of molecularly imprinted polymer (MIP) nanoparticles, the in silico design of MIPs and MIP-based sensors, and two chapters examining the development of sensors from an industrial point of view. The particular focus of all chapters is on practical aspects, either in the development process or the applications of the synthesized materials. This book will serve as an important reference work for business leaders and technology experts in the sensors and diagnostics sector.
This book reflects the current knowledge and trends in the field of biosensors for environmental monitoring. Biosensors combine the power of microelectronics with the selectivity and sensitivity of biological components to detect individual substances or groups of substances in the environment. Biosensors can also be used for recognizing biological effects such as genotoxicity, immunotoxicity and endocrine responses. For this purpose, components of biological origin such as microorganisms, enzymes, receptors or specific nucleic acid sequences are applied for the recognition and combined with a transducer system to provide toxicologic or pharmacologic data within seconds. This approach is expected not only to provide a significant contribution to measurement technology but also a basis for competent political decisions.
Gain the basic knowledge you need to utilize modern technologies for biosensors--quickly and efficiently Biosensors have become virtually indispensable components in the analytical scientist's toolkit. Increasingly, researchers are called upon to design and adapt them for customized applications. Yet, surprisingly, most young scientists graduate without having acquired an integrated working knowledge of the cross-disciplinary principles underlying biosensing strategies. This book was prepared to fill that critical educational gap. Introduction to Bioanalytical Sensors presents to readers a broad view of scientific concepts and principles informing the design and use of biosensing devices and systems. While it offers an integrated presentation of foundational information from an array of related scientific disciplines, the emphasis throughout is on applications rather than theory. Only as much basic science is covered as is necessary for an analytical scientist to use or customize biosensing devices for fulfilling experimental objectives. Major topics covered include: * Biosensors and bioanalytical challenges * Designing for performance * Developing bioselective layers * Fundamentals of electroanalytical sensors * Optically based energy transduction * Thermal and acoustic-wave transduction Introduction to Bioanalytical Sensors, written as a self-teaching guide, assumes that readers have only a bachelor's degree in chemistry or a related field utilizing analytical and physical chemistry, and biochemistry.